The biophysical properties of human skin, such as hydration, elasticity, sebum content, and transepidermal water loss (TEWL), are influenced by both age and body location. These factors play a critical role in dermatological health and the efficacy of skincare products. Aging is generally associated with decreased hydration, elasticity, and sebum production, while TEWL increases, indicating a decline in skin barrier function. Additionally, different anatomical locations exhibit distinct biophysical characteristics due to varying densities of sebaceous glands and exposure to environmental factors. For instance, sebaceous gland-rich areas like the forehead show higher sebum content, while the palms exhibit lower hydration levels. This review presents a comprehensive analysis of current research on the effects of age and body location on skin biophysical properties to guide future dermatological studies and skincare product development.