Burns and wounds destroy the physical skin which is the body’s natural barrier to the external environment. As a result the burned area is prone to infection and colonization by microorganisms. The most common pathogenic colonizing bacterium is Staphylococcus aureus . In order to solve the problem of bacterial resistance to antibiotics, some medicinal plants have been used so as to determine their effectiveness against S. aureus . Among these plants, we have herein used the alcoholic extract of berberine. Our results suggest that the alcoholic extract of berberine, at a concentration 250 mg/mL, can exert a high inhibitory efficacy against S. aureus .